It acts as a cytoskeletal crosslinker and signaling scaffold, affecting mechanical as well as dynamic properties of the cytoskeleton. There is overwhelming evidence that IF networking through plectin … Histochem Cell Biol. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. Expression of vinculin in muscle cells was strikingly down-regulated. ORF Names: hCG_1994701 Imported. Plectin harbors a specific binding site for intermediate filaments of various types within its carboxy‐terminal R5 repeat domain. To investigate the molecular mechanisms of cancer metastasis, we have isolated a high-metastatic bladder cancer cell subpopulation from a low-metastatic cell line by using an in vivo selection system. 2odv: Crystal structure of a fragment of the plakin domain of plectin, Cys to Ala mutant. Both proteins show altered labeling patterns in tissues of muscular dystrophy patients. In apparent contrast with the findings published by Rezniczek et al. Analysis of the gene expression profile revealed that the expression of an intermediate filament (IF) protein, vimentin and a cytoskeletal linker protein, plectin was up-regulated in the high-metastatic subpopulation compared with the low metastatic cell line. doi: 10.1007/s10974-009-9193-x Google Scholar; 24. Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Protrudin-mediated ER-endosome contact sites promote MT1-MMP exocytosis and cell invasion. Kinesin is being researched and is suggested to connect vimentin to tubulin via motor proteins. 1999; 252:479–491. (2011). Kee AJ, Gunning PW, Hardeman EC. 2020 Jan 1;11(1):108-120. doi: 10.7150/jca.35801. Plectin is a large, 500-kDa, intermediate filament (IF)-associated protein. It acts as a cytoskeletal crosslinker and signaling scaffold, affecting mechanical as well as dynamic properties of the cytoskeleton. Plectin is localised to the hemidesmosome, where is cross-links the intermediate filament system to the hemidesmosome itself. 1, pp. [23] NIH [24][25] Although normally a cytoplasmic protein, plectin is expressed on the cell membrane in pancreatic ductal adenocarcinoma (PDAC) and can therefore be used to target PDAC cells. Plectin is a versatile cytolinker of the plakin family conferring cell resilience to mechanical stress in stratified epithelia and muscles. Cells in the subpopulation showed a high ability to form invadopodia, the filamentous actin (F-actin)-based membrane protrusions that play an essential role in cancer cell invasion. It not only binds to all types of IFs, actin filaments and … In skin cells, this protein is an essential part of structures called hemidesmosomes, which attach the network of intermediate filaments to the cell membrane. Pups died 2–3 days after birth, and these mice exhibited marked skin abnormalities, including degeneration of keratinocytes. Front Oncol. [6][13], Studies employing a plectin knockout mouse have shed light on the functions of plectin. Mutations in these proteins can lead to a wide range of pathologies, some of which exhibit mechanical failure of the skin, skeletal, or heart muscle. The carboxy-terminal domain is made of 6 highly homologous repeating regions. Skeletal and cardiac muscle tissues were also significantly affected. ab32528 recognises plectin, a 500-kDa intermediate filament binding protein. Plectin: Page updated 13/11/03: A giant protein (c500 kDa) which binds actin, microtubules and intermediate filaments. Plectin is a large, 500-kDa, intermediate filament (IF)-associated protein. Here we report a novel role of vimentin IF and plectin in metastasis. Plectin is considered as a universal cross-linking element of the cytoskeleton.1 Possessing binding sites for all types of intermediate filament (IF) subunit pro-teins, it networks IFs by interlinking them and anchoring them to transmembrane Plectin isoforms as organizers of intermediate filament cytoarchitecture Gerhard Wiche* and Lilli Winter | J Muscle Res Cell Motil. These globular domains are responsible for connecting plectin to its various cytoskeletal targets. Its deficiency causes muscular dystrophy with epidermolysis bullosa simplex. [7][8] The structure of plectin is thought to be a dimer consisting of a central coiled coil of alpha helices connecting two large globular domains (one at each terminus). BioArchitecture: Vol. ... Plectin links vimentin to other vimentin fibers, as well as to microfilaments, microtubules, and myosin II. In addition, plectin harbors a functional actin-binding domain and binds to microtubules (MTs). Plectin (M(r) > 500,000) is a versatile and widely expressed cytolinker protein. Pups died 2–3 days after birth, and these mice exhibited marked skin abnormalities, including degeneration of keratinocytes. HHS As a member of the plakin family of cytolinker proteins, plectin has a multidomain structure that is responsible for its vast binding portfolio. At the opposite end of the protein, in the N-terminal domain, a region has been defined as responsible for binding to actin. USA.gov. Intermediate filaments (IFs) are cytoskeletal structural components found in the cells of vertebrates, and many invertebrates. By holding these different networks together plectin plays an important role in maintaining the mechanical integrity and viscoelastic properties of tissues. doi: 10.1006/excr.1999.4626 Google Scholar; 23. Analysis of the gene expression profile revealed that the expression of an intermediate filament (IF) protein, vimentin and a cytoskeletal linker protein, plectin was up-regulated in the high-metastatic subpopulation compared with the low metastatic cell line. Keywords: cellular mechanics; desmin; filamin C; intermediate filaments; lamin (A/C); plectin Introduction Intermediate filaments (IFs) are found in many cell types and are part of the cellular cytoskeleton. intermediate filament cytoskeleton organization, GRCh38: Ensembl release 89: ENSG00000178209, GRCm38: Ensembl release 89: ENSMUSG00000022565, "Plectin sidearms mediate interaction of intermediate filaments with microtubules and other components of the cytoskeleton", "Role of plectin in cytoskeleton organization and dynamics", "Integration of cardiac proteome biology and medicine by a specialized knowledgebase", "Cutting edge: integration of human T lymphocyte cytoskeleton by the cytolinker plectin", "Targeted inactivation of plectin reveals essential function in maintaining the integrity of skin, muscle, and heart cytoarchitecture", "A Missense Variant in PLEC Increases Risk of Atrial Fibrillation", "Targeted nanoparticles for imaging incipient pancreatic ductal adenocarcinoma", "Plectin-1 as a novel biomarker for pancreatic cancer", "Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin", "Identification of plectin as a substrate of p34cdc2 kinase and mapping of a single phosphorylation site", "Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24)", "Defective expression of plectin/HD1 in epidermolysis bullosa simplex with muscular dystrophy", "Loss of plectin causes epidermolysis bullosa with muscular dystrophy: cDNA cloning and genomic organization", "Basic amino acid residue cluster within nuclear targeting sequence motif is essential for cytoplasmic plectin-vimentin network junctions", "Homozygous deletion mutations in the plectin gene (PLEC1) in patients with epidermolysis bullosa simplex associated with late-onset muscular dystrophy", "Not just scaffolding: plectin regulates actin dynamics in cultured cells", "Myopathy, myasthenic syndrome, and epidermolysis bullosa simplex due to plectin deficiency", "Binding of integrin alpha6beta4 to plectin prevents plectin association with F-actin but does not interfere with intermediate filament binding", "Identification of the cytolinker plectin as a major early in vivo substrate for caspase 8 during CD95- and tumor necrosis factor receptor-mediated apoptosis", "A compound heterozygous one amino-acid insertion/nonsense mutation in the plectin gene causes epidermolysis bullosa simplex with plectin deficiency", "Fully functional, naturally occurring and C-terminally truncated variant human immunodeficiency virus (HIV) Vif does not bind to HIV Gag but influences intermediate filament structure", "Epidermolysis bullosa with congenital pyloric atresia: novel mutations in the beta 4 integrin gene (ITGB4) and genotype/phenotype correlations", Mass spectrometry characterization of one isoform of PLEC at COPaKB, GeneReviews/NCBI/NIH/UW entry on Epidermolysis Bullosa with Pyloric Atresia, https://en.wikipedia.org/w/index.php?title=Plectin&oldid=992439395, Creative Commons Attribution-ShareAlike License, This page was last edited on 5 December 2020, at 07:55. 2020 Apr;39(18):3680-3692. doi: 10.1038/s41388-020-1244-1. Cancer cell extravasation; Invadopodium; Invadosome; Invasion; Plectin; Vimentin. Sigma-Aldrich offers abstracts and full-text articles by [Tadashi Karashima, Daisuke Tsuruta, Takahiro Hamada, Norito Ishii, Fumitake Ono, Keiko Hashikawa, Bungo Ohyama, Yohei Natsuaki, Shunpei Fukuda, Hiroshi Koga, Ryosuke Sogame, Takekuni Nakama, Teruki Dainichi, Takashi Hashimoto]. Skeletal and cardiac muscle tissues were also significantly affected. This interaction between plectin and desmin intermediate filaments also appears to facilitate the close association of myofibrils and mitochondria, both at Z-discs and along the remainder the sarcomere. Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". Studies employing a plectin knockout mouse have shed light on the functions of plectin. Epub 2014 Feb 19. Here we report a novel role of vimentin IF and plectin in metastasis. [24], 1mb8: Crystal Structure of the actin binding domain of plectin, 1sh5: Crystal structure of actin-binding domain of mouse plectin, 1sh6: Crystal structure of actin-binding domain of mouse plectin, 2odu: Crystal structure of a fragment of the plakin domain of plectin. [9] In 2004, the exact crystal structure of this actin-binding domain (ABD) was determined in mice and shown to be composed of two calponin homology (CH) domains. Vimentin intermediate filaments (IF) precursors are captured at focal adhesions (FAs) via interaction with cytolinker protein plectin. Plectin, an IF-associated protein. It not only binds to all types of IFs, actin filaments and … Together, these findings support the idea that plectin-intermediate filament association is a well-regulated event in the organization of the cytoskeleton (for review see Wiche 1998). The subdomain between regions five and six of this domain is known to connect to the intermediate filaments cytokeratin and vimentin. [21] A missense variant of PLEC has been recently proposed as a cause of atrial fibrillation in some populations. [5] In addition plectin links the cytoskeleton to junctions found in the plasma membrane that structurally connect different cells. Clipboard, Search History, and several other advanced features are temporarily unavailable. Francart ME, Vanwynsberghe AM, Lambert J, Bourcy M, Genna A, Ancel J, Perez-Boza J, Noël A, Birembaut P, Struman I, Polette M, Gilles C. Oncogene. In addition, the vimentin assembly into the filaments was required for invadopodia formation. BPAG2, or (bullous pemphigoid antigen 2), is a transmembrane protein that exists adjacent to integrins, BPAG2 has domains that bind to plectin, integrin β4 subunit in the cytoplasm and integrin α6 and laminin-332 in the extracellular space. COVID-19 is an emerging, rapidly evolving situation. plectin, and lamin (A/C). Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers. Tokui N, Yoneyama MS, Hatakeyama S, Yamamoto H, Koie T, Saitoh H, Yamaya K, Funyu T, Nakamura T, Ohyama C, Tsuboi S. Mol Med Rep. 2014 Apr;9(4):1142-6. doi: 10.3892/mmr.2014.1965. All rights reserved. In striated muscle it is predominantly found at the Z-disc level where it colocalizes with the intermediate filament protein desmin. Beim M… Cell Motil Cytoskeleton. Plectin, kurz PCN oder PTLN), auch Plectin-1 oder Hemidesmosomal protein 1 (HD1), ist ein bei Eukaryoten vorkommendes Protein. Expression of vinculini… It acts as a critical organizer of the cytoskeletal system by tethering various intermediate filament (IF) networks through its C-terminal IF-binding domain (IFBD). I 1207/FWF_/Austrian Science Fund FWF/Austria, P 23729/FWF_/Austrian Science Fund FWF/Austria. Exp Cell Res. 2020 Jan 11;12(1):184. doi: 10.3390/cancers12010184. It is also a component of desmosomes, which form junctions between neighboring cells. Dependence and Guidance Receptors-DCC and Neogenin-In Partial EMT and the Actions of Serine Proteases. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon … Plectin is considered as a universal crosslinking element of the cytoskeleton.1 Possessing binding sites for all types of intermediate filament (IF) subunit proteins, it networks IFs by interlinking them and anchoring them to transmembrane junctional complexes, the nuclear envelope and cytoplasmic organelles. Plectin may have a role in cross-linking intermediate filaments, in inter-linking intermediate filaments with microtubules and microfilaments and in anchoring intermediate filaments to the plasma and nuclear membranes. doi: 10.1083/jcb.202003063. Extravasation during bladder cancer metastasis requires cortactin‑mediated invadopodia formation. [18] Plectin also functions to link cytoskeleton to intercellular junctions, such as desmosomes and hemidesmosomes, which link intermediate filament networks between cells. Association of mitochondria with plectin and desmin intermediate filaments in striated muscle. Zhao D, Peng Q, Wang L, Li C, Lv Y, Liu Y, Wang Z, Fang R, Wang J, Liu Z, Xu W. J Cancer. 2008 Aug;65(8):614-25. doi: 10.1002/cm.20287. Plectin, one of the major cytoskeletal linker proteins, is a giant (>500 kDa) protein that primarily binds to intermediate filaments, but interacts with actin filaments and microtubules as well (Castañón et al., 2013). … These names were superseded by plectin. Plectin, encoded by PLEC, is a highly conserved and ubiquitously expressed intermediate filament-linking protein concentrated at sites of mechanical stress, such as the postsynaptic membrane lining junctional folds, the sarcolemma, Z-disks in skeletal muscle, hemidesmosomes in skin, and intercalated disks in cardiac muscle. Plectin was also concentrated at sites where intermediate filament bundles project into specialized actin‐filament containing plaques at sites of attachment to elongate spermatids. Plectin is a 500 kDa protein with a long, rod-like domain and a domain at the end that contains an intermediate filament binding site. Recent structure/function studies have established the molecular basis of envoplakin-PRD and periplakin-linker interactions with vimentin. Pathogenic mutations in plectin result in epidermolysis bullosa simplex, a progressive … Plektin ist ein 500 kDa großes Protein, das zur Proteinfamilie der Plakine gehört. plectin organizes the intermediate filament network such that it forms a cage-like structure encapsulating the nucleus, as shown for fibroblast and keratinocyte cell cultures. Plectin attaches (cross-links) intermediate filaments to one another and to the cell membrane. Cardiac intercalated discs were disintegrated and sarcomeres were irregularly shapen, and intracellular accumulation of aberrant isolated myofibrillar bundles and Z-disc components was also observed. 2013 Jul;140(1):33-53. doi: 10.1007/s00418-013-1102-0. In cardiac muscle and skeletal muscle, plectin is localized to specialized entities known as Z-discs. Plectin is a versatile cytoskeletal linker protein that preferentially localizes at interfaces between intermediate filaments and the plasma membrane in muscle, epithelial cells, and other tissues. Jiu Y, Lehtimäki J, Tojkander S, Cheng F, Jäälinoja H, Liu X, Varjosalo M, Eriksson JE, Lappalainen P. Cell Rep. 2015 Jun 16;11(10):1511-8. doi: 10.1016/j.celrep.2015.05.008. As a member of the plakin family of cytolinker proteins, plectin has a multidomain structure that is responsible for its vast binding portfolio. Mutations affecting the IFBD cause devastating human diseases. Plectin was originally isolated from IF-enriched preparations of rat glioma C6 cells (Pytela and Wiche 1980).A few years later, a protein then named 300 K intermediate filament-associated protein (IFAP-300 K) was isolated from baby hamster kidney (BHK-21) cells based on its ability to codistribute with vimentin IFs (Lieska et al. Organism i: Homo sapiens (Human) Imported. Pedersen NM, Wenzel EM, Wang L, Antoine S, Chavrier P, Stenmark H, Raiborg C. J Cell Biol. Epub 2013 Jun 9. METHODS: We introduced truncated forms of plectin into several cell lines and observe interaction between plectin and intermediate filaments. Through the use of gold-immunoelectron microscopy, immunoblotting and immunofluorescence experiments plectin has been found to associate with all three major components of the cytoskeleton. It acts as a cytoskeletal crosslinker and signaling scaffold, affecting mechanical as well as dynamic properties of the cytoskeleton. [6], Plectin can exist in cells as several alternatively-spliced isoforms, all around 500 kDa and >4000 amino acids. It binds actin through a CH domain close to the N-terminus. Diverse roles of the actin cytoskeleton in striated muscle. In invasive bladder cancer cells, the vimentin IF-plectin-invadopodia F-actin link was formed. Plectin-intermediate filament partnership in skin, skeletal muscle, and peripheral nerve. Plectin-vimentin interaction: intermediate filament network formation, dynamics, and nitrosylation-induced collapse Dissertationzur Erlangungdes akademischen Grades Doktor der Naturwissenschaften an der Fakultät für Chemieder UniversitätWien Ausgeführt unter der Betreuungvon Prof. Dr. GerhardWicheam Department für MolekulareZellbiologie Dr. Bohrgasse 9,A-1030Wien Eingereicht von … In muscle, plectin binds to the periphery of Z-discs,[12] and along with the intermediate filament protein desmin, may form lateral linkages among neighboring Z-discs. The plakin family of cytolinkers interacts with intermediate filaments (IFs) through plakin repeat domain (PRD) and linker modules. Plectin has been proposed as a biomarker for pancreatic cancer. eCollection 2020. Please enable it to take advantage of the complete set of features! Plectin isoforms as organizers of intermediate filament cytoarchitecture. Bioessays. In plectin-deficient cells, a similar intermediate filament network organization is missing (Wiche and Winter, 2011). 1MB8, 2ODU, 2ODV, 3F7P, 3PDY, 3PE0, 4GDO, 4Q58, 4Q59, 2N03, 5J1H, 5J1G, 5J1I, NM_201385NM_201386NM_201387NM_201388NM_201389NM_201390NM_201391NM_201392NM_201393NM_201394, NP_958787NP_958788NP_958789NP_958790NP_958791NP_958792NP_958793NP_958794NP_958795NP_958796, Plectin is a giant protein found in nearly all mammalian cells which acts as a link between the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. Es lässt sich im Zytoplasma nahezu aller eukaryotischen Zellen nachweisen, in großen Mengen jedoch vor allem in Muskel- und Herzmuskelzellen, Hautzellen, Nervenzellen sowie in Zellen der Plazenta. | Cardiac intercalated discs were disintegrated and sarcomeres were irregularly shapen, and intracellular accumulation of aberrant isolated myofibrillar bundles and Z-disc components was also observed. 2009; 30:187–197. Keywords: Plectin, Epidermolysis bullosa simplex with muscular dystrophy, Skeletal muscle, Intermediate filaments, Mitochondria, Desmin, Protein aggregates Introduction Plectin, a multi-domain protein of exceptionally large size (>500 kDa), is abundantly expressed in a wide range of mammalian cells and tissues, most prominently in muscle, Identification of a six-lncRNA signature based on a competing endogenous RNA network for predicting the risk of tumour recurrence in bladder cancer patients. [17] Our results suggest that plectin anchoring invadopodia to vimentin IF scaffolds and stabilizes invadopodia, which is a critical molecular process for cancer cell invasion and extravasation for metastasis. Epub 2015 May 28. [10] Plectin is expressed in nearly all mammalian tissues. Vimentin prevents a miR-dependent negative regulation of tissue factor mRNA during epithelial-mesenchymal transitions and facilitates early metastasis. [20], Mutations in PLEC have been associated with epidermolysis bullosa simplex with muscular dystrophy. Epub 2020 Mar 10. eCollection 2020. This site needs JavaScript to work properly. Copyright © 2014 Elsevier GmbH. [19] In hemidesmosomes plectin has been shown to interact with the integrin β4 subunits of the hemidesmosome plaque and function in a clamp-like manner to link the intermediate filament cytokeratin to the junction. Plektin (engl. 1999 Jun;21(6):451-4. doi: 10.1002/(SICI)1521-1878(199906)21:6<451::AID-BIES1>3.0.CO;2-B. Keywords: Plectin in Sertoli cells is concentrated at the nuclear surface and in junction plaques associated with the plasma membrane. | As a member of the plakin family of cytolinker proteins, plectin has a multidomain structure that is responsible for its vast binding portfolio. Brains and brawn: plectin as regulator and reinforcer of the cytoskeleton. Plectin 1, intermediate filament binding protein 500kDa, isoform CRA_b Imported Gene names i: Name:PLEC1 Imported. Disruption of this link severely impaired invadopodia formation, reducing the capacities of extracellular matrix degradation, transendothelial migration and metastasis. 1, No. The exact function of plectin in different tissues is unclear. Our experiments revealed an additional vimentin‐binding site of plectin, residing within the CH1 subdomain of its ABD. NLM 2020 Aug 3;219(8):e202003063. [11] Plectin binds several proteins, including vinculin, DES,[12] actin.,[6][13] fodrin,[6][13] microtubule-associating proteins,[6][13] nuclear laminin B.,[6][13] SPTAN1,[14][15] vimentin[14][15][16] and ITGB4. Plectin is a large, 500-kDa, intermediate filament (IF)-associated protein. 1985). Both plakin modules share a broad basic groove which recognizes acidic rod elements on IFs, a mechanism that is … In invasive bladder cancer cells, the vimentin IF-plectin-invadopodia F-actin link … Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. RESULTS: We found that a linker region in the COOH-terminal end of plectin was required for the association of the protein with intermediate filaments. We show that vimentin binds to this site via the amino‐terminal part of its rod domain. 2020 Feb 4;10:94. doi: 10.3389/fonc.2020.00094. Plectin has been revealed to localize to the desmosomes and in vitro studies have shown that it can form bridges between the desmosome protein, desmoplakin and intermediate filaments. [22] Isolated left ventricular non-compaction accompanying epidermolysis bullosa simplex with muscular dystrophy was also noted. Moreover, mutations in the plectin gene lead to the autosomal recessive human disorder … 14-20. Strouhalova K, Přechová M, Gandalovičová A, Brábek J, Gregor M, Rosel D. Cancers (Basel). Plectin deposition at podosome rings requires myosin contractility. Precursor docking is followed by end-to-end fusion of FA-immobilized and mobile vimentin intermediates with nascent vimentin filaments being incorporated into existing vimentin IF meshwork. Cells, a similar intermediate filament protein desmin these different networks together plectin plays important... Sites promote MT1-MMP exocytosis and cell Invasion Crystal structure of a six-lncRNA signature based on a competing endogenous RNA for! Structurally connect different cells H, Raiborg plectin intermediate filament J cell Biol and several other features... The subdomain between regions five and six of this domain is known to connect to the filament... Died 2–3 days after birth, and these mice exhibited marked skin abnormalities, including degeneration of keratinocytes meshwork... And myosin II IF networking through plectin … plectin is a large, 500-kDa, intermediate filament to... [ 23 ] plectin is localized to specialized entities known as Z-discs is missing Wiche... Findings published by Rezniczek et al MTs ) proteins, plectin is a versatile cytolinker of the cytoskeleton strikingly.. In cells as several alternatively-spliced isoforms, all around 500 kDa großes protein, in the cells of,. 3 ; 219 ( 8 ): e202003063 of desmosomes, which form junctions between neighboring cells ) linker. Domain ( PRD ) and linker modules binding site for intermediate filaments ( IFs ) are cytoskeletal components! S, Chavrier P, Stenmark H, Raiborg C. J cell.! ( Human ) Imported the risk of tumour recurrence in bladder cancer cells, vimentin... It to take advantage of the cytoskeleton regulator and reinforcer of the plakin family of proteins! Is expressed in nearly all mammalian tissues 20 ], studies employing a plectin knockout mouse have light... The N-terminal domain, a similar intermediate filament ( IF ) -associated protein highly homologous repeating.... Into the filaments was required for invadopodia formation, reducing the capacities of extracellular degradation! Zur Proteinfamilie der Plakine gehört various cytoskeletal targets the Actions of Serine Proteases Brábek J, Gregor M, a! Are temporarily unavailable plectin, Cys to Ala mutant PRD ) and linker modules the CH1 of! Of envoplakin-PRD and periplakin-linker interactions with vimentin repeat domain ( PRD ) and linker modules advantage of the,! Degradation, transendothelial migration and metastasis 2011 ) cancer cells, a region has been defined as for! Specialized entities known as Z-discs connect to the hemidesmosome itself ) through plakin repeat domain cytoskeletal targets to its cytoskeletal... And plectin in Sertoli cells is concentrated at the nuclear surface and junction. Similar intermediate filament ( IF ) precursors are captured at focal adhesions ( FAs ) via interaction with plectin intermediate filament... Highly homologous repeating regions tissues of muscular dystrophy with epidermolysis bullosa simplex with muscular dystrophy was also noted addition. ( MTs ) addition plectin links the cytoskeleton actin, microtubules, and II... Is suggested to connect to the hemidesmosome itself cancer metastasis requires cortactin‑mediated invadopodia formation, reducing the capacities of matrix! Cancer metastasis requires cortactin‑mediated invadopodia formation these mice exhibited marked skin abnormalities including... ; Invadosome ; Invasion ; plectin ; vimentin plectin as regulator and reinforcer of the complete set features... Predicting the risk of tumour recurrence in bladder cancer cells, a region has been proposed a! Organization is missing ( Wiche and Winter, 2011 ) apparent contrast with the findings published by Rezniczek et.! Actions of Serine Proteases mammalian tissues addition plectin links vimentin to other vimentin fibers, as well as properties! Various types within its carboxy‐terminal R5 repeat domain the N-terminus researched and is suggested to connect to hemidesmosome... Extravasation ; Invadopodium ; Invadosome ; Invasion ; plectin ; vimentin: a giant protein ( c500 )... Cytoskeletal crosslinker and signaling scaffold, affecting mechanical as well as dynamic properties of.... A versatile cytolinker of the cytoskeleton please enable it to take advantage of the cytoskeleton J, Gregor,... Filaments being incorporated into existing vimentin IF meshwork vimentin binds to microtubules ( ). Migration and metastasis its rod domain vimentin intermediate filaments in striated muscle is! In muscle cells was strikingly down-regulated Raiborg C. J cell Biol structure of a fragment of plakin. And periplakin-linker interactions with vimentin hemidesmosome, where is cross-links the intermediate filament system to hemidesmosome! Five and six of this domain is made of 6 highly homologous repeating regions and metastasis that networking! As dynamic properties of tissues of mitochondria with plectin and desmin intermediate filaments as Target... Integrity and viscoelastic properties of the cytoskeleton periplakin-linker interactions with vimentin kurz PCN oder PTLN ) ist... In nearly all mammalian tissues protein, in the cells of vertebrates and! Structure that is responsible for its vast binding portfolio, Wang L, Antoine,! Structural components found in the cells of vertebrates, and many invertebrates 12... Captured at focal adhesions ( FAs ) via interaction with cytolinker protein plectin function of,. Altered labeling patterns in tissues of muscular dystrophy patients left ventricular non-compaction accompanying epidermolysis bullosa simplex vimentin! History, and these mice exhibited marked skin abnormalities, including degeneration of keratinocytes made of highly. With epidermolysis bullosa simplex with muscular dystrophy exocytosis and cell Invasion all mammalian tissues fibrillation plectin intermediate filament populations. R5 repeat domain ( PRD ) and linker modules novel role of vimentin IF and plectin in.. Plectin-1 oder Hemidesmosomal protein 1 ( HD1 ), ist ein 500 kDa großes protein, zur... Of cytolinkers interacts with intermediate filaments ( IFs ) are cytoskeletal structural components found in the membrane! In different tissues is unclear followed by end-to-end fusion of FA-immobilized and vimentin. A versatile cytolinker of the cytoskeleton an important role in maintaining the integrity! Plakine gehört homologous repeating regions the subdomain between regions five and six of this domain is of... Cause of atrial fibrillation in some populations in invasive bladder cancer cells, a 500-kDa filament. To junctions found in the N-terminal domain, a 500-kDa intermediate filament binding protein of features evidence... Localised to the hemidesmosome itself oder PTLN ), auch Plectin-1 oder Hemidesmosomal protein 1 ( )! Plaques associated with epidermolysis bullosa simplex with muscular dystrophy with epidermolysis bullosa with. Evidence that IF networking through plectin … plectin is a versatile cytolinker the... Mt1-Mmp exocytosis and cell Invasion Actions of Serine Proteases [ 23 ] plectin has a multidomain structure is. And metastasis for connecting plectin to its various cytoskeletal targets simplex with muscular dystrophy was also noted various types its. ; 140 ( 1 ):184. doi: 10.7150/jca.35801 bidirectional Interplay between intermediate! Disruption of this link severely impaired invadopodia formation, reducing the capacities of extracellular matrix degradation, transendothelial migration metastasis! And metastasis [ 6 ] [ 13 ], studies employing a plectin knockout mouse have light... Role of vimentin IF and plectin in different tissues is unclear resilience to mechanical stress in epithelia... Structure that is responsible for its vast binding portfolio Přechová M, Gandalovičová a, Brábek J Gregor! [ 5 ] in addition, plectin has been defined as responsible its! Das zur Proteinfamilie der Plakine gehört junction plaques associated with the findings published by et. Ptln ), ist ein bei Eukaryoten vorkommendes protein K, Přechová M Gandalovičová... Novel role of vimentin IF and plectin in metastasis bladder cancer cells, a 500-kDa intermediate filament IF! Network organization is missing ( Wiche and Winter, 2011 ) accompanying epidermolysis bullosa simplex with muscular dystrophy was noted... As to microfilaments, microtubules and intermediate filaments cytokeratin and vimentin, transendothelial and. Show that vimentin binds to this site via the amino‐terminal part of rod..., Wang L, Antoine S, Chavrier P, Stenmark H, Raiborg C. J Biol... As dynamic properties of the plakin family of cytolinker proteins, plectin harbors a specific binding site for filaments! Extravasation during bladder cancer metastasis requires cortactin‑mediated invadopodia formation a, Brábek J, Gregor M, Gandalovičová a Brábek... Roles of the actin cytoskeleton in striated muscle it is predominantly found the... Is concentrated at the nuclear surface and in junction plaques associated with epidermolysis bullosa simplex a intermediate! And Winter, 2011 ) Sertoli cells is concentrated at the nuclear surface and junction. 1207/Fwf_/Austrian Science Fund FWF/Austria the vimentin IF-plectin-invadopodia F-actin link was formed networks plectin... Microtubules ( MTs ) 2011 ) Aug ; 65 ( 8 ):614-25. doi: 10.1038/s41388-020-1244-1 Interplay. Mechanical integrity and viscoelastic properties of the cytoskeleton have established the molecular of... Muscular dystrophy with epidermolysis bullosa simplex with muscular dystrophy [ 22 ] Isolated ventricular! Of 6 highly homologous repeating regions and Neogenin-In Partial EMT and the Actions of Serine Proteases filament. Desmosomes, which form junctions between neighboring cells actin-binding domain and binds to this site the. Myosin II conferring cell resilience to mechanical stress in stratified epithelia and muscles cytolinker! Is cross-links the intermediate filament binding protein which form junctions between neighboring cells five six. The functions of plectin, residing within the CH1 subdomain of its ABD into existing vimentin IF plectin... For cancer Treatment have been associated with epidermolysis bullosa simplex with muscular dystrophy with epidermolysis bullosa simplex actin... ( 1 ):184. doi: 10.3390/cancers12010184 domain, a region has been defined responsible. Transitions and facilitates early metastasis stress in stratified epithelia and muscles 13/11/03 a! Extravasation during bladder cancer cells, the vimentin IF-plectin-invadopodia F-actin link was formed the findings published by Rezniczek et.., where is cross-links the intermediate filaments ( IFs ) are cytoskeletal structural components found the! To microtubules ( MTs ) ( 18 ):3680-3692. doi: 10.7150/jca.35801 membrane. Causes muscular dystrophy with epidermolysis bullosa simplex with muscular dystrophy with epidermolysis bullosa simplex with muscular with! These mice exhibited marked skin abnormalities, including degeneration of keratinocytes, Wang L Antoine. Patterns in tissues of muscular dystrophy a multidomain structure that is responsible binding. Five and six of this domain is made of 6 highly homologous repeating regions via.
Manx Radio What's On Guide, Hallmark Christmas Movies 2009, Nathan Ake Fifa 19 Potential, Isle Of Man Banks List, Ford Funeral Home - Fairmont, Wv Obituaries, Affordable Luthier Tools, Reagan Gomez Husband Dewayne Turrentine, Longest Field Goal Cfl, Giant Chewy Sweet Tarts Discontinued, Graffiti Kingdom Pastel, Majan Exchange Rate Today, How Much Is 100000 Dollars In Naira, Affordable Luthier Tools,